Fig. 2: Catalytic properties of SgUGT94-289-3 on mogrosides.

a UPLC chromatographic curves of each single glucosylation step catalyzed by SgUGT94-289-3 towards different mogrosides, with the type of mogroside substrates and the reaction time labeled in the right. The peak of target product of each reaction is indicated by a black arrow. Mogrosides (peaks) containing the same number of glucoses are shown with the same background color. b Histogram of conversion efficiency of the target product using M2E, M3, M3E, M4A or SIA as substrates, calculated from the curves in (a). c Dynamics of products converted in an in vitro continuous glucosylation assay using M2E as the starting substrate. Time course: 720 min. SIA is not included in the calculation due to its very low production (yield of 2%). d Histogram of products’ conversion rate in different time points (0, 40, 300 and 720 min), calculated from the curves in (c). Conversion rate of each product (%) is represented by the percentage of corresponding product in total products. Data are presented as mean values ± SD (n = 3 biological replicates). The negative control group consisted of buffer without enzyme or substrates. Source data are provided as a Source Data file.