Table 5 Extraction and sequencing details for the archival mouse specimens

From: Century-old chromatin architecture revealed in formalin-fixed vertebrates

Mouse specimen

Tissue mass (mg)

DNA yield (ng/mg tissue)

Raw read pairs (M)

Reads mapping (%)

Mean insert size (bp)

Mean genome coverage (X)

Lab1

287

14.4

250

67

129

15.3

Lab2

545

7.8

199

68

124

11.9

Lab3

593

7.6

228

65

124

13.1

Wild1

467

4.5

239

67

117

13.3

Wild2

624

6.8

263

67

118

14.9

Wild3

763

3.5

227

68

112

12.2

  1. Summary of the extraction and sequencing results for processing available archival liver tissue from six mouse specimens with MNase treatment. We report tissue mass processed in mg, DNA yield in ng/mg of tissue, the number of raw read pairs (millions), the percentage of raw reads mapping to the reference genome, the mean insert size of mapped reads in base pairs and the mean genome coverage after de-duplication and GC correction.
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