Fig. 5: APE1-probe for afterglow/MRI imaging of radiation dose-dependent APE1 activity in tumor. | Nature Communications

Fig. 5: APE1-probe for afterglow/MRI imaging of radiation dose-dependent APE1 activity in tumor.

From: Imaging-guided companion diagnostics in radiotherapy by monitoring APE1 activity with afterglow and MRI imaging

Fig. 5

a Scheme illustration of tumor-targeted imaging of APE1-probe (containing tumor-targeted AS1411 aptamer) or imaging of APE1-probe (no targeting, uncontaining AS1411 aptamer), and radiation dose-dependent afterglow/MRI imaging in vivo. b Afterglow imaging of mice i.v. injected with APE1-probe (no targeting) (n = 3 mice per group). c Fluorescence images of mice i.v. injected with APE1-probe (no targeting) (n = 3 mice per group). d−g Afterglow images of the mice treated with various radiation doses (0–6 Gray) and then intravenously injected with APE1-probe (n = 3 mice per group). h Quantified afterglow signal intensity. i Signal-to-noise ratio of afterglow imaging and fluorescence imaging of the mice intravenously injected with APE1-probe (no targeting). j T1 and T2 MRI images of the mice intravenously injected with APE1-probe (no targeting) (n = 3 mice per group). k–q The mice were treated with various radiation doses (0−6 Gray) and then i.v. injecting APE1-probe for afterglow and MRI imaging (n = 3 mice per group). k–n T1 and T2 MRI images. o, p Quantified T1 and T2 MRI signal intensity from (j–n). q Subtracted ΔT1–ΔT2 value of MRI signal intensity (ΔT1: subtracted T1 signal; ΔT2: subtracted T2 signal). r Scheme illustration and L&T-ESI images of mice with 6 Gray radiation treatment and APE1-probe injection. s signal-to-noise ratio comparison of MRI images before or after L&T-ESI treatment. t, u L&T-ESI images and quantification of tumor with various radiation dose treatment (n = 3 mice per group). v, w L&T-ESI images and quantification of mice with 6 Gray of radiation dose treatment at different time points (n = 3 mice per group). All afterglow imaging were obtained with white light irradiation (10 mW/cm2) time of 10 s and acquisition time of 60 s. Fluorescence imaging was obtained with excitation wavelength of 605 nm and emission of Cy5.5 channel. Data are presented as means ± SD. Statistical significance was determined using two-tailed Student’s t-test for pairwise comparisons, and one-way ANOVA analysis of variance for multiple groups. p values > 0.05 were considered non-significant, while p values < 0.05 were considered statistically significant. Source data are provided as a Source Data file.

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