Fig. 7: Afterglow/MRI imaging of APE1 activity in radiation-induced liver injury.
a Scheme illustration for APE1-activated afterglow/MRI imaging for evaluating radiation-induced liver organ injury. b−i Liver region of mice was treated with various radiation dose (0−6 Gray) and then injected with APE1-probe (n = 3 mice per group). b, c Afterglow luminescence images and increased signal intensity. d, e Afterglow images and signal intensity of in-vitro liver organ after injection of APE1-probe for 8 h. f T1 MRI and T2 MRI images of liver region of mice treated with various radiation dose (0−6 Gray). g, h Increased T1 MRI intensity and decreased T2 MRI intensity. i Subtracted MRI signal intensity (Δ T1–ΔT2 value). j L&T-SEI images of the liver region with various radiation treatment (at 2-h post-monitoring) (n = 3 mice per group). k Quantified signal intensity of L&T-SEI images (Δ T1–ΔT2 value), data from (j). All afterglow images were obtained with a white light irradiation (10 mW/cm2) time of 10 s and an acquisition time of 60 s. Data are presented as means ± SD. Statistical significance was determined using two-tailed Student’s t-test for pairwise comparisons, and one-way ANOVA analysis of variance for multiple groups. p values > 0.05 were considered non-significant, while p values < 0.05 were considered statistically significant. Source data are provided as a Source Data file.
