Fig. 2: RSV infection blocks cholesterol egress from lysosomes by reducing LAL activity. | Nature Communications

Fig. 2: RSV infection blocks cholesterol egress from lysosomes by reducing LAL activity.

From: Cholesterol-rich lysosomes induced by respiratory syncytial virus promote viral replication by blocking autophagy flux

Fig. 2

HEp-2 cells were either mock-infected or infected with RSV (MOI = 1) in the presence or absence of orlistat (10 μM) for the indicated durations. a, b The activity of LAL in HEp-2 cells 24 h after RSV infection was determined using flow cytometry (n = 3 independent experiments). c The mRNA level of LAL gene in HEp-2 cells 12 h or 24 h after RSV infection was determined using RT-PCR (n = 3 independent experiments). d, e Western blotting analysis of LAL in HEp-2 cells 12 h or 24 h after RSV infection (n = 3 independent experiments). Data are shown as the mean ± SD, statistical analysis using two-sided Student’s t-test (c, e) or one-way ANOVA (b) (**P < 0.01 and ****P < 0.0001 compared to the blank control group).

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