Fig. 6: Correlation analysis between P300-mediated H3K18cr modification and transcripts.
From: P300 regulates histone crotonylation and preimplantation embryo development
a Schematic of sample collection for H3K18cr CUT&Tag analysis in control, P300 knockdown (P300-KD), and P300-I1394G or NaCr rescue embryos. b Pie chart showing the distribution of H3K18cr peaks across annotated genomic regions in control, P300-KD, P300-I1394G rescue, and NaCr rescue embryos at late 8-cell stages. c Enrichment profiles show the fluctuation of H3K18cr signals in control, P300-KD, P300-I1394G rescue, and NaCr rescue embryos at late 8-cell stages. d Combination Column and Bubble Charts show the difference in total H3K18cr modification peaks (n = 25209) among control, P300-KD, P300-I1394G rescue, and NaCr rescue embryos at the late 8-cell stages. Data are presented as mean values ± SEM. n = 2 biological replicates. Statistical analysis was performed using two-tailed unpaired t-tests. e Comparison of the occupancy of H3K18cr around the TSS region (TSS ± 2.5 kb) in control, P300-KD, and P300-I1394G or NaCr rescue embryos. f Boxplots showing the signals of the H3K18cr promoter region (two biological replicates) at global genes of different experiment groups. Control (n = 7934), P300-KD (n = 3674), P300-KD + I1394G (n = 10175), P300-KD + NaCr (n = 11181). g–i The plot displays the cumulative frequency of log2 fold changes in FPKM among control (g, target n = 7553, non-target n = 9690), P300-I1394G rescue (h, target n = 9697, non-target n = 8037), NaCr rescue (i, target n = 10448, non-target n = 6667), and P300 knockdown embryos. A noticeable shift to the left for H3K18cr target genes suggests an overall positive alteration in gene expression. n = 2 biological replicates. j Track views display the enrichment of H3K18cr peaks and RNA-seq signals among control, P300-KD, and P300-I1394G or NaCr rescue embryos. The vertical axis corresponds to normalized tag counts for H3K18cr peaks and RNA-seq in each sample. Refgene refers to the genome data from NCBI and IGV. In (d), (f), (g), (h), and (i), Statistical analysis was performed using two-tailed unpaired t-tests. The center line and edges of the boxes represent the median and quartiles of the data points, respectively. The minimum and maximum values in the boxplots correspond to quartile 1 − 1.5 × interquartile range and quartile 3 + 1.5 × interquartile range, respectively.
