Fig. 1: α-Tomatine degradation assay by LC-MS. | Nature Communications

Fig. 1: α-Tomatine degradation assay by LC-MS.

From: Botrytis cinerea combines four molecular strategies to tolerate membrane-permeating plant compounds and to increase virulence

Fig. 1

A LC-MS profiles of liquid cultures of wild type isolate B05.10 (highlighted in gray) and various knockout mutants upon incubation with 200 µM α-tomatine. B LC-MS profiles of liquid cultures of wild type isolate M3a (highlighted in gray) and various complemented mutants upon incubation with 200 µM α-tomatine. C LC-MS profile of α-tomatine upon incubation with purified BcTOM1 protein for 2 h. D Structures of the metabolites 1–6. Note that the commercial α-tomatine used as substrate contained traces of dehydrotomatine (peak 2), which differs in the aglycone moiety, but is also a substrate for tomatinases.

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