Fig. 2: Differences between B. cinerea isolates B05.10 and M3a in sensitivity to saponins, in virulence on plant leaves and in genomic composition. | Nature Communications

Fig. 2: Differences between B. cinerea isolates B05.10 and M3a in sensitivity to saponins, in virulence on plant leaves and in genomic composition.

From: Botrytis cinerea combines four molecular strategies to tolerate membrane-permeating plant compounds and to increase virulence

Fig. 2

A Mycelium growth on plates containing 400 µM α-tomatine and 30 µM digitonin, colony diameters were measured after 3 days. The mock treatment for experiments with α-tomatine was methanol, while for experiments with digitonin, it was water. Data are presented as mean values ± standard deviation (SD) of all biological replicates. B–D Infection of B05.10 and M3a on tomato (B), Digitalis purpurea (C) or Nicotiana benthamiana (D). Disease incidence represents the proportion of inoculation droplets that resulted in expanding lesions. Open dots represent individual values of disease incidence or lesion diameter, based on three-to-four independent inoculation assays. Data are presented as mean values ± SD of all biological replicates. P-values of two-tailed Student’s t-tests are provided above the bars. Source Data are provided as a Source Data file. E Illustration of the absence of genes Bcin08g00060 and Bcin08g00070 in isolate M3a. Red and blue boxes represent gene models in B05.10 and M3a, respectively, with the gene IDs plotted above or below the box. Dotted lines join orthologous genes. TE, transposable element in M3a. Start and end coordinates are plotted at the extremities of the contigs. Annotations of the genes in panel (E) are provided in Supplementary Table S1A.

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