Fig. 5: Testing the frugal CRISPRkit using in-house cell-free system.
From: A frugal CRISPR kit for equitable and accessible education in gene editing and synthetic biology
a Experimental data showing the performance of single-color CRISPRkit reactions using in-house CFS. Three biological replicates calculated from mean of two technical replicates are plotted for each group. The bars represent the mean of biological replicates, and the error bars represent the standard deviation. Two-tailed Student’s t-test was performed; p values are indicated above the no guide and guide condition bars. b, c Experimental data showing dual-color CRISPRkit reactions using in-house CFS, measured by a fluorescent plate reader (b) or smartphone images and analyzed by CRISPectra (c). From left to right, each group of bars (red – eforRed, yellow – fwYellow) show eforRed single-color positive control, fwYellow single-color positive control, eforRed + fwYellow dual-color positive control, dual-color with eforRed-targeting sgRNA (sgRed), dual-color with fwYellow-targeting sgRNA (sgYellow), dual-color with both eforRed- and fwYellow-targeting sgRNAs, and negative control (water only). For b, c, ten biological replicates are plotted for each group. The bars represent the mean of biological replicates, and the error bars represent the standard deviation. Two one-way ANOVA were performed; p values generated by comparison of chosen conditions to no-guide control via Dunnett’s multiple comparisons test are indicated above the chosen condition, above the fold of repression. Source data are provided as a Source Data file.
