Fig. 6: SA enhances RxLR23KM-triggered plant defense response by suppressing ERD15La activity.
The activity of NbERD15La was affected by SA or PAC. A Transcript levels of NbERD15La were measured by qRT-PCR after treatment with SA or PAC. Values are presented as mean ± SD, n = 3 independent experiments. B Expression of NbERD15La was detected using quantitative western blots in leaves following treatment with SA or PAC. Quantification of NbERD15La expression was calculated as relative intensity of NbERD15La bands. Protein loading is indicated by Ponceau S. Values are presented as mean ± SD, n = 3 independent experiments. C Neither SA nor PAC affected the interaction of RxLR23KM with NbERD15La. Right panels showed expression (+) of NbERD15La-GFP, RxLR23KM-FLAG, and GFP. Left panels showed the combination of RxLR23KM and NbERD15La. The ratio of the band intensity of IP to input was shown in the bottom of IP panel. Equal protein loading was indicated by Ponceau S. D–F N. benthamiana treatment with single or sequential combinations of RxLR23KM and SA in TRV:NbERD15La (S) or TRV:GFP (NS) plants. NS or S plants without any treatment were used as control. D Representative lesions were taken under UV irradiation at 48 h after P. capsici infection. Mean diameter of lesions was measured at 48 hpi. Values are presented as mean ± SD, n = 30 samples. E qPCR was used to measure the relative biomass on the ratios of P. capsici to S and NS leaves DNA at 48 hpi. The ratio of NS leaves without any treatment was assigned to value 1.0. Values are presented as mean ± SD, n = 3 independent experiments. F Expression levels of PR1/2 was detected by qRT-PCR at 48 hpi. Values are presented as mean ± SD, n = 3 independent experiments. The data in (B, D–F) were analyzed by ANOVA one-way comparison followed by least significant difference (LSD) test and different letters above the bars indicate a significant difference at p < 0.05. Pcβ-actin and NbEF1α were identified as the most suitable reference genes for normalization in P. capsici and N. benthamiana, respectively. These experiments (B–D) were repeated three times. Source data are provided as a Source Data file.
