Fig. 6: Integrative analysis of target sequencing and proteome.

A Genetic variants of 295-gene panel in 53 primary HGSOC (PDS-HGSOC) patients and 43 relapsed HGSOC (RLP-HGSOC) patients. The associations between gene mutations and chemoresistance were performed by Fisher’s exact test: **, 0.001 ≤ p-value < 0.01. B The volcano plot showed comparison of proteome between chemosensitive HGSOC patients with HRR mutations and chemoresistant ones without any HRR mutations by two-sided unpaired Welch’s t test. The colored dots represent DEPs. The orange dots represent 33 DEPs which were annotated to have direct interactions with 14 genes in the HRR pathway, and those with significant p-value or fold change were labeled with gene names. HRR, homologous recombination repair; WT, patients without gene mutations in HRR pathway. C The overlapping relationship of chemoresistance associated DEPs between primary and relapsed HGSOC cohorts. PDS-HGSOC, primary HGSOC patients; RLP-HGSOC, relapsed HGSOC patients. D The most enriched network using DEPs between chemoresistant and chemosensitive groups of relapsed HGSOC cohort. P-values are derived from one-sided Fisher’s Exact Test for pathway enrichment. The green and red patterns represent downregulated and upregulated proteins in chemosensitive groups, while the blue ones were predicted to be inhibited by IPA. The shape of each molecule is according to legends of IPA. E The heatmap showed the expression of DNA damage repair associated DEPs between chemosensitive and chemoresistant groups of primary and relapsed patients by two-sided unpaired Welch’s t test. The outliers of protein expression were defined as those with absolute values of Z-score larger than 2.5, and these outliers were imputed as 2.5 in the heatmap. Source data are provided as a Source Data file.