Fig. 4: Mo/MΦ adopt a pro-repair profile in response to exogenous Tregs.

a–c Endogenous cardiac Mo/MΦ from saline- and Treg-treated groups were sorted and sequenced on days 4 and 7 post-MI (n = 4 for saline, n = 3 for Tregs). The experimental design is shown in (a). b Heat map of selected significantly upregulated and downregulated DEGs (FDR < 0.05) depicting standardised gene expression values on days 4 and 7 post-MI (individual biological replicates are shown). Genes are classified according to function with the coloured tab to the left of the heat map. c GO enriched terms depicting biological processes in the significantly upregulated and downregulated genes in Mo/MΦ following exogenous Treg treatment from days 4 and 7 post-MI (FDR < 0.01, adjusted by Benjamini-Hochberg correction). d, e LCA ligation was performed in wild-type mice, followed by a systemic injection of either saline or Tregs 1 day post-MI. Hearts were collected on day 7 post-MI and stained for F4/80 (green) to identify Mo/MΦs, with proteins of interest (CD206, LYVE1, CD163, and SPARC) in red, and nuclei stained in blue. d Representative images. Scale bar: 100 μm. e Quantification of signal intensity expressed as average raw density per F4/80⁺ cells (n = 4). For all graphs, Boxes show median (centre line) and interquartile range (edges), whiskers show the range of values and dots represent individual data points. Two-tailed Student’s t-test was used to compare the groups in (e). P values are indicated. Panel (a) created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).