Fig. 4: The crRNA stem-loop length regulates transposition activity.
From: Distinct horizontal transfer mechanisms for type I and type V CRISPR-associated transposons
A We tested changes in the direct repeat sequence, stem (green), loop (blue), and handle lengths (5'-orange, 3'-yellow). B The effect of each feature on the integration efficiency. Data are shown as mean ± S.D., n = 3 biologically independent experiments. Black: DR from the native CAST CRISPR array; gray: a sequence-scrambled DR that preserved the wild-type stem loop structure; and other colors correspond to the schematic in (A).
