Fig. 6: The ELKS1 condensate guides the growth of cortical MTs through CLASP2.

a Distribution of ELKS1 condensates along MTs. Cellular regions with less dense MTs were chosen for imaging. Similar phenomena were observed in more than three experiments. b Kymographic analysis showing the MT plus end with the CLIP170 condensate passing through multiple ELKS1 condensates in live cells. Three snapshots indicated by arrowheads in the kymography are shown aside. See also Movie S3. c Schematic diagrams illustrating the quantitative evaluation of MT distribution patterns by applying 2D Fourier transformation. d Cellular analysis of cortical MT organization regulated by CLASP2. Cells were fixed with methanol at −20 °C. 2D Fourier transform was applied to MT patterns of different samples, and corresponding results were shown at the bottom. e Quantification of the ratio of the major axis to the minor axis for the ellipses in the frequency domain as shown in the bottom panels in (d). Four regions near the cell edge in each cell were selected as the ROIs. The error bars represent the values in the 5%–95% range. The middle, bottom, and top of boxplots represent, respectively, the median, the 25th percentile, and the 75th percentile (n = 80 ROIs from 20 cells).