Fig. 3: Phylogenomic affiliation of the MAGs, AcrAs and McrAs.
From: Thermophilic Hadarchaeota grow on long-chain alkanes in syntrophy with methanogens
a phylogenomic affiliation of the Hadarchaeota MAGs based on 37 conserved protein sequences and using 233 representative archaeal genomes. b phylogenomic affiliation of the AcrAs and McrAs based on the alignments with 461 aligned positions. The tree branches were classified into four distinct groups: the ACR clade; TACK clade; Class I&II clade and Class III clade, the last three are canonical McrA clades. ACR clade contains the AcrAs of Ca. Melinoarchaeum spp.; Class I&II clade contains the McrAs of Methanothermobacter and Methanothrix. Alignments were generated using MAFFT43 and then filtered with trimAl44, and the trees were built by the IQ-Tree45 method with the model LG + C60 + F + G with 1000 bootstrap replicates. Bootstrap values > 90% shown in gray dots.
