Fig. 6: Validation of Prap1 expression in human placental cells.
From: Single-cell RNA sequencing reveals placental response under environmental stress
Expression of Prap1 in human placental cell lines (BeWo, HTR-8/SVneo, and SWAN71) exposed to arsenic measured by (a–c) qRT-PCR (normalized to GAPDH) or (d) western blotting. n = 3 cell culture plates in each group. Unpaired, two-tailed t-tests (e) Effect of PRAP1 on LDH release in BeWo cells with or without arsenic treatment (n = 3 cell culture plates in each group; Unpaired, two-tailed t-tests). Effect of PRAP1 knockdown on (f) cell number measured by Cyquant assay (n = 15 cell culture plates in each group; Two-way ANOVA followed by Tukey’s test adjusted for multiple comparisons), (g) cell proliferation measured by EdU incorporation (n = 12 cell culture plates in each group; Unpaired, two-tailed t-tests), (h) cell death measured by PI staining in BeWo cells (n = 13 cell culture plates in each group; Unpaired, two-tailed t-tests). As arsenic. Source data are provided as a Source Data file.
