Fig. 3: Identification of nanobody recognizing TraN via the CAM discovery platform.

a Stacked bar plot illustrating the changes in the relative abundance of individual nanobodies within the population across three rounds of bio-panning to enrich for bacteria displaying TraN-binding nanobodies. Sequences were discerned through the CDR regions of the nanobody gene. The relative abundances of all unique nanobodies identified are shown, and the respective counts are detailed in Supplementary Data 1–3. Colors denote the ranking of nanobody abundance from the highest to lowest. b Macroscopic aggregation analysis to assess cell-cell adhesion. Cultures of E. coli strains displaying TraN or Nb^traN were incubated either individually or in combination. The OD₆₀₀ of the supernatant after the settling period is shown below the image. c) The amino acid sequence of Nb^traN, shows CDR1, CDR2, and CDR3 in green, blue, and orange, respectively. d, e The ITC binding profiles of Nb^traN with TraN^surf at 25 °C. f Crystal structure of Nb^traN (yellow) in complex with TraN^surf (blue) (PDB: 8X7N). CDR1, CDR2, and CDR3 are presented in green, blue, and orange, respectively. g A magnified view of the interacting residues is indicated between Nb^traN and TraN^surf. See also Supplementary Table 1. Source data are provided as a Source Data file.