Fig. 6: Mounting NbompA and NbompC on PICs directs antibacterial activity toward target E. coli in bacterial mixtures.
From: A whole-cell platform for discovering synthetic cell adhesion molecules in bacteria
a Schematic of target killing of bacteria (navy) in a complex bacterial population by PICs (pink). Survival rate of E. coli strains (wild-type, ΔompA, ΔompC, and ΔrfaD) upon incubation with PICs displaying NbompA (b), NbompC (c), non-matched control (Ctrl) (d), or NbBamA (e) in liquid medium. Survival rate of wild-type E. coli when incubated with PICs expressing either NbompA (f) or NbompC (g). Various starting ratios of PICs to E. coli were tested: 1:10; 1:5; 1:2.5; 2.5:1; 5:1; 10:1; and 100:1. E. coli target (wild-type) and non-target (ΔompA or ΔompC) survival rates following treatment with PICs producing NbompA (h) or NbompC (i). Prior to incubation, target and non-target E. coli were mixed in ratios of 1:1, 1:10, and 1:100. j Survival rate of wild-type E. coli within a mouse fecal bacterial population before and after incubation with PICs displaying NbompA, NbompC, or NbBamA. Data are presented as means ± SD. n = 3 biological replicates with three technical replicates each. Asterisks indicate statistically significant differences between the indicated mean values (p < 0.05, two-tailed t-test). See also Supplementary Fig. 10–12. Source data are provided as a Source Data file.
