Fig. 6: Controlled respiro-fermentative glycerol conversion to isobutanol by the NNmini ΔldhA +glpD + pIBA strain. | Nature Communications

Fig. 6: Controlled respiro-fermentative glycerol conversion to isobutanol by the NNmini ΔldhA +glpD + pIBA strain.

From: Engineering new-to-nature biochemical conversions by combining fermentative metabolism with respiratory modules

Fig. 6

A Schematic representation of glycerol fermentation to isobutanol. By deleting the native lactate synthesis route (ΔldhA), the metabolic flux can be re-directed towards isobutanol. The genes for isobutanol synthesis are expressed from the pIBA plasmid (optimized pIBA441). The isobutanol pathway scheme was adapted from Ghosh et al. 41. B Growth of the NNmini ΔldhA +glpD + pIBA strain grown with glycerol as sole carbon source with and without IPTG. Here, acetate served as a negative control in addition to the “no IPTG” condition. C Fermentation products detected in the supernatants of the NNmini ΔldhA + glpD + pIBA strain grown with different glycerol concentrations. The mean values of triplicate measurements are shown with standard deviation. Source data are provided as a Source Data file.

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