Table 1 Strains and plasmids used in this study
Name | Description/deletion | Reference |
|---|---|---|
E. coli strain | ||
DH5α | Cloning of overexpression constructs | |
SIJ488 | WT carrying integrated λ-red recombinase and flippase | |
ΔubiCA | ΔubiCA | This study |
NNQ | Δndh ΔnuoEFG ΔubiCA | This study |
NNmini | ∆ndh ∆nuoEFG ∆putA ∆glcDEF ∆dld ∆mqo ∆dadA ∆poxB ∆kefF ∆wrbA ∆fadE ∆yieF ∆mdaB/ygiN ∆glpD ∆gpsA ∆lldD ∆glpABC ∆sdhABCD ∆lhgO | This study |
NNmini + glpD | ∆ndh ∆nuoEFG ∆putA ∆glcDEF ∆dld ∆mqo ∆dadA ∆poxB ∆kefF ∆wrbA ∆fadE ∆yieF ∆mdaB/ygiN ∆lldD ∆gpsA ∆glpABC ∆sdhABCD ∆lhgO | This study |
NNmini + glpD ΔldhA | ∆ndh ∆nuoEFG ∆putA ∆glcDEF ∆dld ∆mqo ∆dadA ∆poxB ∆kefF ∆wrbA ∆fadE ∆yieF ∆mdaB/ygiN ∆lldD ∆gpsA ∆glpABC ∆sdhABCD ∆lhgO ∆ldhA | This study |
JW0999 | KEIO ΔputA | |
JW2121 | KEIO Δdld | |
JW2198 | KEIO Δmqo | |
JW1178 | KEIO ΔdadA | |
JW0855 | KEIO ΔpoxB | |
JW0045 | KEIO ΔkefF | |
JW0989 | KEIO ΔwrbA | |
JW5020 | KEIO ΔfadE | |
JW3691 | KEIO ΔyieF | |
JW3389 | KEIO ΔglpD | |
JW3580 | KEIO ΔlldD | |
JW2635 | KEIO ΔlhgO | |
Plasmid | ||
pIBA4 | Expression plasmid encoding the iBuOH pathway [pBBRori-aadA1(SpR)-TL-lacI-PlacIq] (814bb)-PT7A1-O34-RBSAS7-alsSBsu-RBSAC7-ilvCEco-6E6-RBSAD5-ilvDEco-RBSAK7-kivdLla-RBSAA2-adhALla-M5-gfp-TP22 | |
