Table 3 Parameter settings for LCMS measurement targets
Name | Precursor ion | Product ion | Collision energy [V] | Fragmentor voltage [V] | Cell accelerator voltage [V] | Dwell time [msec] | Polarity |
|---|---|---|---|---|---|---|---|
NAD | 664.1 | 524 428 | 18 26 | 380 380 | 5 5 | 50 50 | Positive Positive |
NADH | 666.1 | 649 514 | 17 23 | 380 380 | 5 5 | 50 50 | Positive Positive |
NADP | 744.1 | 604 508 | 20 32 | 380 380 | 5 5 | 50 50 | Positive Positive |
NADPH | 746.1 | 729 135.8 | 17 40 | 380 380 | 5 5 | 50 50 | Positive Positive |
AMP | 346 | 96 78.9 | 24 30 | 380 380 | 5 5 | 50 50 | Negative Negative |
ADP | 425.9 | 327.8 133.9 | 17 22 | 380 380 | 5 5 | 50 50 | Negative Negative |
ATP | 505.9 | 407.9 158.8 | 21 28 | 380 380 | 5 5 | 50 50 | Negative Negative |
