Fig. 7: Tape measure protein (TMP) of the diffocin.
From: Atomic structures of a bacteriocin targeting Gram-positive bacteria
a Sectional view of composite cryoEM density map of the diffocin in the pre-contraction state. TMPs are colored in magenta and the other components are in semitransparent colors. b–e Zoom-in views of diffocin TMP densities as indicated in (a). Corresponding top views are shown on the right. TMP models are fitted into the cryoEM densities in side views. f Interface between the TMP trimer and spike trimer. g Interactions between the C-terminal helix of the TMP and the N-terminal helix of the spike. Residues on the interface are labeled and shown in stick representation. h Model of the full-length diffocin TMP. One TMP subunit is rainbow colored from its N-terminus (blue) to C-terminus (red). i Illustration of predicted secondary structures of TMPs from phages and phage tail-like nanomachines. Alpha-helices, transmembrane (TM) domains and disordered regions were predicted by Phyre231. Globular domains were predicted by AlphaFold225. Globular domains in the same color indicate their structural similarity (see details in Supplementary Fig. 9).
