Fig. 9: Analysis of mDA neuron positioning after in vivo circRmst knockdown.
From: Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development
a Schematic representation of the IUE approach. Transuterine, intracerebroventricular injections with the Cre-dependent reporter iSureCre and a circRmst KD construct (pll3.7-sh-circRmstI) or control plasmid (pll3.7-Scr2) in the E12 vMB of Pitx3cre/+ embryos were followed by transcranial vMB electroporation. At E18, brains were harvested and processed for immunohistochemistry. Figure was generated with icons from BioRender.com. b Representative images of electroporated mDA neurons in the E18 vMB after control or circRmst KD. White arrows indicate the position of electroporated mDA neurons. Boxed region is shown at higher magnification in right hand panels. circRmst KD causes neurons to migrate further away from the midline. Scale bars: left = 200 µm, right = 100 µm. c, d Quantification of the location of electroporated mDA neuron along the y- (dorsal-ventral) and x-axis (medial-lateral). Datapoints represent average per brain. n (pll3.7) = 16 brains, n (pll3.7-sh-circRmstI) = 8 brains, paired two-tailed t-test, mean ± SD, (c) P = 0.2529 (d) *P = 0.0264. IUE in utero electroporation, D dorsal, L lateral, M medial, V ventral. Source data are provided as a Source Data file.
