Fig. 4: Kinetics of primary T cell responses following Delta challenge.
From: Variant-proof high affinity ACE2 antagonist limits SARS-CoV-2 replication in upper and lower airways
NHP (n = 8/group) were challenged with 2 × 105 TCID50 Delta and simultaneously treated with RBD-62 (green circles) or PBS (gray circles). a–e Peripheral blood mononuclear cells (PBMC) or f–h lymphocytes from BAL were collected prior to challenge (immediately preceding challenge for PBMC and 1 month pre-challenge for BAL) and on days 2, 4, 7, 9 and 14 post challenge. Cells were stimulated with WA1 S1 and S2 peptide pools and responses were measured by intracellular cytokine staining (ICS). a, f Percentage of memory CD4+ T cells expressing TH1 markers (IL-2, TNF or IFNγ). b, g Percentage of memory CD4+ T cells expressing TH2 markers (IL-4 or IL-13). c, h Percentage of memory CD8+ T cells expressing IL-2, TNF or IFNγ. d, e Percentage of TFH cells expressing CD40L or IL-21, respectively. Dotted lines set at 0%. Reported percentages may be negative due to background subtraction and may extend beyond the lower range of the y-axis. Circles, boxes and horizontal lines represent individual animals, interquartile range and median, respectively, while minima and maxima are denoted at whisker termini. Due to pre-specified minimum cell numbers per sample required for analysis, some timepoints include data from <8 NHP/group. Data are provided as a Source Data file.
