Fig. 2: ARID1A requires both PrLDs and Pfam homology domain to incorporate BAF subunits into condensates.

a Co-immunoprecipitation assay performed to detect the interaction between endogenous BAF complex subunit and ARID1A wild type (WT), ΔDD, or ΔPfam mutant expressed in 293T cells. The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes. b Representative confocal images showing the cellular localization of different GFP-BAF complex subunits. Scale bar: 5 μm. The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes. c Representative confocal images demonstrating the colocalization pattern of recombinant ARID1A proteins (green) and SMARCB1 (red). Scale bar: 5 μm. The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes. d Representative confocal images illustrating the colocalization pattern of recombinant ARID1A proteins (green) and SMARCD1 (red). Scale bar: 5 μm. The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes. e Schematics of ARID1A Corelet system. f Representative confocal images of the Corelet system using PrLD1-mch-SspB or PrLD1-Pfam-mch-SspB to observe recruitment of BAF complex subunit upon blue light stimulation. Scale bars: 5 μm. The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes. g Representative confocal images of 293T cells transfected with recombinant ARID1A and immunostained with anti-SMARCD and anti-SMARCC1 antibodies. Scale bars: 5 μm. The representative images supported by the relevant statistics have been chosen upon three independent preparations with similar outcomes. Source data are provided as a Source Data file.