Fig. 2: B. burgdorferi antigenic peptide library screening and selection of prevalent LD-specific epitopes. | Nature Communications

Fig. 2: B. burgdorferi antigenic peptide library screening and selection of prevalent LD-specific epitopes.

From: Rapid single-tier serodiagnosis of Lyme disease

Fig. 2

a Overview of the screening of epitopes to select the most relevant peptide antigens that function in the paper-based xVFA platform. b Heatmap representing the normalized signal intensities of peptides screened against patient samples positive (+) and negative (−) for LD, utilized in the development of the paper-based multiantigen xVFA platform. c Variance in the normalized signal intensity for each antigen peptide, determined through xVFA screening of control patient sera. The variance is plotted in descending order of reactivity, illustrating the comparative activity of the peptides against both positive and negative patient serum samples, highlighting the most active antigens. d Heatmap representing the correlation of peptides against each other. Inset shows examples of highly correlated peptides (Rec164 and OspC1) and non-correlated peptides (modVlsE-FlaB and OppA4), which yield additional information for a diagnostic panel. Panel a was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).

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