Table 2 Comparison of the two-tier and single-tier xVFA assay with gold standard serology tests
Conventional laboratory tests | Previous assay [19] | Current assay | ||||
|---|---|---|---|---|---|---|
Two-tier test (STTT) | Two-tier test (MTTT) | Two-tier xVFA | Single-tier xVFA | |||
Assay specifications | Test format | Two-tier | Two-tier | Two-tier | Single-tier | |
Antigen type used | Proteins | Proteins/peptide | Proteins/peptide | Peptide | ||
IgM or IgG detection | IgM and IgG individually | IgM and IgG individually | IgM and IgG individually | Combined IgM/IgG | ||
Point of care? | No | No | Yes | Yes | ||
Cost | >$400¶ | >$400¶ | $25.98 | $2.59 | ||
Performance | Bay Area Lyme Disease Biobank | Sensitivity | NA | NA | 90.5% | 95.5% |
Specificity | NA | NA | 87.0% | 100.0% | ||
CDC Lyme Disease Biobank | Accuracy | 88% | 91% | NA | 88.0% | |
