Fig. 2: General enzymatic synthesis of Lewisx glycofluoroforms. | Nature Communications

Fig. 2: General enzymatic synthesis of Lewisx glycofluoroforms.

From: Synthesis and screening of a library of Lewisx deoxyfluoro-analogues reveals differential recognition by glycan-binding partners

Fig. 2: General enzymatic synthesis of Lewisx glycofluoroforms.

Mass spectrometry-based screening was performed using ITag derivatives before scaling up the synthesis of the azidopropyl glycans for preparation of neoglycolipid (NGL) and polyhydroxyethylacrylamide (PHEA) derivatives for use in binding assays. UDP Uridine diphosphate, ATP Adenosine 5’-triphosphate, GTP Guanosine 5’-triphosphate, DBCO-DH dibenzocycloctyne-functionalised DHPE, DHPE 1,2-Dihexadecyl-sn-glycero-3-phosphoethanolamine.

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