Fig. 5: Glyconanoparticle-based sensing of CTB.
a Polymer-tethered glyconanoparticles; b Principle of detection due to gold nanoparticle red-blue shift upon aggregation with CTB; c UV-Visible spectra for gold nanoparticles with native Lewisx (LeX1) and fluorinated LeX22; d Dose-dependent response of library of Lewisx glyconanoparticles to CTB. Data is presented as mean normalised Abs700 from UV-Visible spectroscopy ± standard deviation of 3 replicates. Control experiments are shown in Supplementary Fig. 17a–c. e Correlation of glycan array and glyconanoparticle binding data LeX22, which had a glycan array binding score of 55 ± 53, is omitted from the correlation graph as binding was too weak to be quantified in the nanoparticle assay. Pearson correlation analysis: r(7) = −0.96, p = 0.000041 (two-sided). Error bars on the x-axis correspond to values of duplicate measurements, and error bars on the y-axis correspond to the fitting uncertainty reported for EC50 values from the data shown in Fig. 5d. The gold nanoparticle binding data generated in this study have been deposited in the University of Manchester Figshare database (https://figshare.manchester.ac.uk).
