Fig. 2: Lipid classes that change in the liver or plasma of mice in normal and Dlk1-manipulated pregnancy. | Nature Communications

Fig. 2: Lipid classes that change in the liver or plasma of mice in normal and Dlk1-manipulated pregnancy.

From: A co-ordinated transcriptional programme in the maternal liver supplies long chain polyunsaturated fatty acids to the conceptus using phospholipids

Fig. 2: Lipid classes that change in the liver or plasma of mice in normal and Dlk1-manipulated pregnancy.

A–J Grouped relative abundance of lipid classes that are significantly different in liver or plasma in pregnant mice (15.5 dpc) compared to virgin controls (A–H), and in pregnant mice that lack fetal or maternal-derived DLK1 protein (I–J). Significance was only considered if identified in at least two genotype-matched replicate group comparisons. All class data are found in Supplementary Data Tables S1–2. Data is presented as boxplots (median and IQR (25th and 75th percentiles) with whiskers showing 1.5*IQR) with individual values. Two-way ANOVA with Sidak’s multiple comparisons test was performed to determine significant class shifts between experimental groups (*p-value < 0.05; **p-value < 0.01; ***p-value < 0.001; ****p-value < 0.0001). Statistical tests were performed independently per ionisation mode and per genotype-matched replicate comparison. p-values for A: 1vs2 = 1.60 × 10−05, 6vs8 = 1.00 × 10−15, 3vs5 = 8.36 × 10−10; B: 1vs2 = 1.20 × 10−07, 6vs8 = 0.0002, 3vs5 = NS; C: 1vs2 = 6.54 × 10−05, 6vs8 = 1.00 × 10−15, 3vs5 = 5.00 × 10−15; D: 1vs2 = 7.50 × 10−14, 6vs8 = 8.34 × 10−10, 3vs5 = 8.70×10−14; E: 1vs2 = 0.0074, 6vs8 = 2.01 × 10−12, 3vs5 = 5.82×10−05; F: 1vs2 = 7.43 × 10−06, 6vs8 = 1.00 × 10−15, 3vs5 = 1.00 × 10−15; G: 1vs2 = NS, 6vs8 = 1.54 × 10−06, 3vs5 = 0.028; H: 1vs2 = NS, 6vs8 = 1.00 × 10−15, 3vs5 = 0.0009; I: 8vs7 = 8.39 × 10−05, 5vs4 = 3.00 × 10−13; J: 8vs7 = 2.61 × 10−05, 5vs4 = 5.82 × 10−07. Plasma data: n = 6 (group 1), n = 7 (group 2), n = 7 (group 3), n = 5 (group 4; +ve mode) n = 8 (group 4; −ve mode), n = 6 (group 5), n = 5 (group 6), n = 8 (group 7), n = 6 (group 8; +ve mode), n = 7 (group 8; −ve mode); Liver data: n = 7 (group 1), n = 6 (group 2), n = 7 (group 3), n = 8 (group 4), n = 8 (group 5), n = 7 (group 6), n = 7 (group 7, +ve mode), n = 8 (group 7; −ve mode), n = 8 (group 8); mice per group. CL cardiolipin, LPC lyso-phosphatidylcholine, LPE lyso-phosphatidylethanolamine, LPS lyso-phosphatidylserine, PC phosphatidylcholine, PC-O PC plasmalogen, PE phosphatidylethanolamine, TG triglyceride. Source data are provided as a Source Data file.

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