Table 1 PC and PE species of interest that were identified as candidate biomarkers associated with pregnancy

From: A co-ordinated transcriptional programme in the maternal liver supplies long chain polyunsaturated fatty acids to the conceptus using phospholipids

Saturation (# double bonds)

Lipid

Fatty Acid Composition (isoform abundance)

Compartment

CBM (Y/N)

Change in Pregnancy

4 or more

PC(37:4)

17:0/20:4 (% undetermined)

Plasma

Y

UP

17:0/20:4 (% undetermined)

Liver

Y

UP

4 or more

PC(38:6)

16:0/22:6 (85%)

Plasma

Y

UP

16:0/22:6 (66%)

Liver

Y

UP

4 or more

PC(39:6)

17:0/22:6 (60%)

Plasma

Y

UP

17:0/22:6 (85%)

Liver

Y

UP

4 or more

PC(40:6)

18:0/22:6 (95%)

Plasma

Y

UP

18:0/22:6 (95%)

Liver

Y

UP

4 or more

PE(40:6)

18:0/22:6 (85%)

Plasma

Y

UP

18:0/22:6 (85%)

Liver

Y

UP

3 or less

PE(34:2)

16:0/18:2 (100%)

Plasma

N

n/a

16:0/18:2 (100%)

Liver

Y

DOWN

3 or less

PE(36:2)

18:0/18:2 (85%)

Plasma

N

n/a

18:0/18:2 (75%)

Liver

Y

DOWN

3 or less

PC(34:2)

16:0/18:2 (95%)

Plasma

Y

DOWN

16:0/18:2 (95%)

Liver

N

n/a (trending down)

3 or less

PC(36:3)

18:1/18:2 (55%)

Plasma

Y

DOWN

18:1/18:2 (50%); 16:0/20:3 (50%)

Liver

Y

DOWN

3 or less

PE(34:1)

did not analyse

Plasma

N

n/a

did not analyse

Liver

Y

DOWN

  1. PC and PE candidate biomarkers (CBMs) that distinguish pregnant groups (15.5 dpc; groups 2, 8 and 5) from virgin groups (groups 1, 6 and 3) were selected based on their level of unsaturation and the tissue compartment in which they were identified. sn−1/sn−2 fatty acid compositions were assigned using the most abundant isoform identified from targeted LC-MS/MS analysis in plasma and liver (percentage of total lipid/glyceride signal indicated in brackets; see Supplementary Data Table S6). CBMs were classified as lipids that passed both Bonferroni-adjusted two-tailed t-tests (liver threshold, p = 0.00234; plasma threshold, p = 0.00283) and sparse partial least squares discriminant analysis in at least two genotype-matched virgin vs pregnant comparisons (see Supplementary Data Table S4 for full CBM list). Individual CBM plots are depicted in Supplementary Fig. S3. All CBM tests were performed independently per ionisation mode and per genotype-matched replicate comparison. Plasma data: n = 7 (group 1), n = 7 (group 2), n = 7 (group 3), n = 6 (group 5), n = 5 (group 6), n = 7 (group 8); Liver data: n = 8 (group 1; +ve mode), n = 7 (group 1; −ve mode), n = 7 (group 2), n = 7 (group 3; +ve mode), n = 8 (group 3; −ve mode), n = 8 (group 5), n = 7 (group 6), n = 8 (group 8). PC phosphatidylcholine, PE phosphatidylethanolamine. Source data are provided as a Source Data file.
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