Fig. 7: The IgC domain of PD-L1 interacts with Ku at the core domain. | Nature Communications

Fig. 7: The IgC domain of PD-L1 interacts with Ku at the core domain.

From: PD-L1 deglycosylation promotes its nuclear translocation and accelerates DNA double-strand-break repair in cancer

Fig. 7

a Schematic representation of structural WT and various deletion mutants of PD-L1 and Ku80 proteins. b, c HA-tagged WT Ku80 was co-transfected with Flag-tagged WT PD-L1 or various PD-L1 deletion mutant(s) into 293T cells, followed by co-IP using anti-HA (b) or anti-Flag antibody (c), respectively. The Ku-associated PD-L1 (b, upper) or PD-L1-associated Ku (c, upper) was analyzed by western blot. IgG or 1/10 input was used as negative or positive control, respectively. d, e Flag-tagged WT PD-L1 was co-transfected with HA-tagged WT Ku80 or various Ku80 deletion mutant(s) into 293T cells, followed by co-IP using anti-Flag (d) or anti-HA antibody (e), respectively. The PD-L1-associated Ku (d, upper) or Ku-associated PD-L1 (e, upper) was analyzed by western blot. IgG or 1/10 input was used as negative or positive control, respectively. Source data are provided as a Source Data file.

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