Fig. 4: Profiling ovarian tumor cells with scTaILoR-seq.
From: Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer
a UMAP visualization of dissociated tumor cells (n = 2482) from a HGSOC patient (P1). Scaled expression of cell-type-specific canonical marker genes are shown as additional UMAPs (right). b Scaled expression of the top-10 differentially expressed transcripts across coarse cell-type groupings. The colored horizontal bar at top corresponds to cell-type annotations in (a). c IL-32 transcript models for theta and beta isotypes and differential IL-32 isoform usage identified across CD8 + T cells and PDGFRa-/b+ Fibroblasts (two-sided Mann–Whitney U test). d Zoomed in UMAP of T cells showing successfully reconstructed TCRs. e Proportion of T cells with TCR chain assignments: no chain identified (N/A), one chain identified (α or β) or both chains identified (α & β). f Higher-order (>2 cells) clonotypes identified within T cells. The inner ring denotes the number of cells while the outer ring denotes individual clonotype frequency. g Projection of cells expressing TP53 chr17:7674241_G > C (HGVS 17:g.7674241 G > C). h Correlation between single-cell MSK HGSOC geneset expression and PROGENy pathway activity scores. i MSK HGSOC Cancer.3 (JAK-STAT/NF-kB/TNF-active) and Cancer.6 (Hypoxia-active) geneset scores mapped to epithelial cell embedding. Source data are provided as a Source Data file.
