Fig. 4: Targeted imaging, self-reporting, and PDT effect of MNB-Pyra Nbs in cells.
a Western blot analysis of the EGFR expression in A431, HeLa, A549, and NIH-3T3 cells. b Cell viability of different cells incubated with MNB-Pyra Nbs at various concentrations in dark (n = 4 experimental replicates). c Quantification of the fluorescence intensity of MNB-Pyra Nbs in different cells (n = 3 experimental replicates). d Flow cytometry analysis of MNB-Pyra Nbs uptake in cells. e ROS generating detection of MNB-Pyra Nbs in A431 cells under normoxia and hypoxia conditions with DCFH-DA (Ex: 488 nm, Em: 500–550 nm) and DHE (Ex: 560 nm, Em: 600–630 nm). f Apoptosis imaging of A431 cells using Annexin V-FITC (Ex: 488 nm, Em: 490–530 nm) and PI (Ex: 488 nm, Em: 600–700 nm) kit. g Cell viability of A431 cells after PDT with MNB-Pyra Nbs under normoxia or hypoxia conditions (n = 4 experimental replicates). h–i MTT assays of MNB-Pyra Nbs and MNB-Pyra dimer in different cells after PDT (n = 4 experimental replicates). j Wound healing rate of different cells after PDT with MNB-Pyra Nbs (n = 3 experimental replicates). k Fluorescence imaging of MNB-Pyra Nbs (0.2 µM) in A431 and HeLa cells with or without light irradiation. l Quantification of the fluorescence intensity in A431 and HeLa cells in figure (n = 3 experimental replicates). m Flow cytometry analysis of fluorescence intensity in cells with or without light irradiation after incubated with MNB-Pyra Nbs (0.2 µM) for 2 h. n Schematic representation of MNB-Pyra Nbs cleaved by ROS in the cells. Schematic diagram (n) created with Figdraw (ID: IIUTAd4f1d) and PowerPoint. Data are shown as mean ± SD (b, c, g, h, i, j, l). Statistical significance in (c, g, h, j, l) was calculated via one tailed Student’s t test. P < 0.05 is considered to be statistically significant. Scale bar = 20 µm. d, e, f, k, m Each experiment was repeated three times with similar results. Source data are provided as a Source Data file.
