Fig. 2: OTUD6 physically Interacts with the 40 S ribosome and opposes 40 S ribosome ubiquitination. | Nature Communications

Fig. 2: OTUD6 physically Interacts with the 40 S ribosome and opposes 40 S ribosome ubiquitination.

From: OTUD6 deubiquitination of RPS7/eS7 on the free 40 S ribosome regulates global protein translation and stress

Fig. 2: OTUD6 physically Interacts with the 40 S ribosome and opposes 40 S ribosome ubiquitination.The alternative text for this image may have been generated using AI.

a Volcano plot of OTUD6C183A physical interactors, n = 3 biological replicates per genotype. b FLAG co-immunoprecipitation with tagged OTUD6C183A followed by western analysis for RACK1 and OTUD6. c Genetic interaction analysis of heterozygous mutations for OTUD6 and Rack1. One way ANOVA with Sidak’s multiple comparisons test. (n = 24, 24, 20, 20, 24, 24) d E2 conjugating enzymes and E3 ligases tested for genetic interactions with OTUD6 for MMS sensitivity. e–g Suppressors of OTUD6C183A MMS sensitivity. One way ANOVA with Sidak’s multiple comparisons test. (e: n = 15, 15, 14, 14. f: n = 20, 20, 20, 19, 13, 15. g: n = 20, 16, 17, 9). Data are presented as mean values +/− SEM. Dots on on bar graphs represent biological replicates. Source data and statistics are provided as a Source Data file.

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