Fig. 2: m1Ψ impacts amino acid selectivity in vitro and in HEK293 cells. | Nature Communications

Fig. 2: m1Ψ impacts amino acid selectivity in vitro and in HEK293 cells.

From: N1-Methylpseudouridine and pseudouridine modifications modulate mRNA decoding during translation

Fig. 2: m1Ψ impacts amino acid selectivity in vitro and in HEK293 cells.The alternative text for this image may have been generated using AI.

A Representative eTLC displaying dipeptide products from translation reactions performed with 70 S initiation complexes (ICs) containing an unmodified UUU or m1ΨUU codon in the A site and total E. coli tRNA aminoacylated with a single amino acid (aa-TC). Relative to ICs containing a UUU codon in the A site, higher levels of miscoded MI and MV dipeptide products and lower levels of MS were generated from m1ΨUU containing ICs. B Summary of amino acid substitutions observed by mass spectrometry in a luciferase peptide incorporated on m1Ψ-containing mRNAs translated in 293H cells (Supplementary Table 8). Source data are provided as Source Data file.

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