Fig. 3: Ѱ and m1Ѱ impact the rates of the ribosome reacting with near-cognate tRNAs in a sequence context dependent manner. | Nature Communications

Fig. 3: Ѱ and m1Ѱ impact the rates of the ribosome reacting with near-cognate tRNAs in a sequence context dependent manner.

From: N1-Methylpseudouridine and pseudouridine modifications modulate mRNA decoding during translation

Fig. 3: Ѱ and m1Ѱ impact the rates of the ribosome reacting with near-cognate tRNAs in a sequence context dependent manner.The alternative text for this image may have been generated using AI.

Plots of dipeptide formation as a function of time (seconds). Miscoding reactions were performed with E. coli ICs containing 35S-fMet-tRNAfMet bound to an AUG start codon in the P site, and unmodified (black circles-UUU) or modified (blue squares-Ψ/m1ΨUU, green diamonds-UΨ/m1ΨU, red triangles-UUΨ/m1Ψ) codons in the A site. Purified ICs were reacted with TCs containing (A) Ile-tRNAIle(GAU), (B) Leu-tRNALeu(CAG)), or (C) Ser-tRNASer(UGA). At least 30 independent time points were collected for each IC in experiments conducted over three or more separate days. D The fitted rate constants (kobs) for isoleucine, leucine, and serine misincorporation on Ψ- and m1Ψ- modified codons relative to the fitted rate constants for isoleucine, leucine, and serine misincorporation on a UUU codon. Each ratio has an n of 1 (since each is computed by dividing kobs,modified/kobs,UUU) and error bars were calculated by propagating the 95% confidence intervals of each fitted rate constant. Source data are provided as Source Data file.

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