Fig. 4: Changes in the energetics of mRNA:tRNA interactions correlate with observed differences in Phe and Ile incorporation on Ψ− and m1Ψ− containing codons. | Nature Communications

Fig. 4: Changes in the energetics of mRNA:tRNA interactions correlate with observed differences in Phe and Ile incorporation on Ψ− and m1Ψ− containing codons.

From: N1-Methylpseudouridine and pseudouridine modifications modulate mRNA decoding during translation

Fig. 4: Changes in the energetics of mRNA:tRNA interactions correlate with observed differences in Phe and Ile incorporation on Ψ− and m1Ψ− containing codons.The alternative text for this image may have been generated using AI.

A Summary of data in Supplementary Tables 4 and 5 displaying how a Ψ and m1Ψ impact the rate constants for the reaction of near-cognate tRNAs. B, C Summary of MM data. Gray bars reflect the change in energy for interactions between a modified mRNA position (Ψ/m1Ψ) and the base paring (B) tRNAPhe(GAA) or (C) tRNAIle(GAU) residue (ΔEbp) relative to an unmodified mRNA U. Black bars reflect the total change in energy (ΔEΣΨ/m1Ψ:X-Y) for interactions between a modified mRNA position (Ψ/m1Ψ) and three (B) tRNAPhe(GAA) or (C) tRNAIle(GAU) residues (the base paired nucleotide, and nucleotides 5′ and 3′ the bp) relative to an unmodified mRNA U. D Molecular model of an unmodified sequence coding for a Phe UUU codon and a tRNAIle(GAU). The hypermodification t6A37 is also shown on the tRNA.

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