Fig. 5: mRNA expression of RIOK2 is reduced in aging individuals.
From: RIOK2 transcriptionally regulates TRiC and dyskerin complexes to prevent telomere shortening
a RIOK2 mRNA in PBMCs from n = 30 young individuals (19–30 years) versus n = 146 nonagenarians ( ≥ 90 years); Data represented as mean ± SEM. b, c Correlation of mRNA levels of RIOK2 with TRiC and Dyskerin complex genes in PBMCs from n = 30 young individuals (19–30 years) and n = 146 nonagenarians ( ≥ 90 years), respectively. d Association of RIOK2 mRNA levels in whole blood with corresponding age of individuals in a sub-sample of the SHIP cohort; n = 987. Linear mixed effect model with age as exposure and RIOK2 transcript level as outcome. e RIOK2 mRNA levels in whole blood samples from n = 232 younger ( < 40 years: minimum: −2.89, 1. quantile – 1.5*IQR: −1.50, 1. quantile: −0.38, median: 0.04, 3. quantile: 0.73, 3. quantile + 1.5*IQR: 1.84, maximum: 2.80) versus n = 249 older ( > 60 years: minimum: −3.22, 1. quantile – 1.5*IQR: −1.89, 1. quantile: −0.66, median: −0.0020, 3. quantile: 0.57, 3. quantile + 1.5*IQR: 1.81, maximum: 3.11) SHIP participants. Linear mixed effect model with age as exposure and RIOK2 transcript level as outcome. f Association of RIOK2 mRNA levels with TRiC complex genes in whole blood samples from a sub-sample of the SHIP cohort; n = 987. Linear mixed effect model with RIOK2 transcript level as exposure and the target gene’s mRNA levels as outcome. g Association of RIOK2 mRNA levels with dyskerin complex genes in whole blood samples from a sub-sample of the SHIP cohort; n = 987. Linear mixed effect model with RIOK2 transcript level as exposure and the target gene’s mRNA levels as outcome. Unpaired non-parametric Mann–Whitney t-test in a; Two-tailed Pearson’s correlation performed in b and c, and Pearson’s correlation coefficients (r) and P values are shown. Linear mixed effect models were used in d–g (detailed statistical analyses in Methods section). When multiple array probes map to the same gene, the results of all probes are reported. Effect sizes (β-values) are only stated for significant probes after Bonferroni correction for multiple testing, i.e. when p < 0.05/7 = 0.0071 for the TRiC complex and p < 0.05/4 = 0.013 for the dyskerin complex. See also Supplementary Fig. 6. Source data are provided as a Source Data file.
