Fig. 8: Overexpressions of α4 and α5 Integrins lead to moderate apicobasal defects and apical extrusions. | Nature Communications

Fig. 8: Overexpressions of α4 and α5 Integrins lead to moderate apicobasal defects and apical extrusions.

From: Modelling variability and heterogeneity of EMT scenarios highlights nuclear positioning and protrusions as main drivers of extrusion

Fig. 8

a–d Representative images for immunostaining on cryosections against atypical protein kinase C (aPKC) from embryos expressing membrane-GFP (a, n = 3), α4-Integrin (b, n = 14), α5-Integrin (c, n = 5) or a combination of α4 and α5 Integrins (d, n = 7). e–h Representative images for immunostaining on cryosections against Laminin from embryos expressing membrane-GFP (e, n = 3), α4-Integrin-GFP (f, n = 14), α5-Integrin-Cherry (g, n = 6) or a combination of both α4 and α5 Integrins (h, n = 7). Nuclei are stained with DAPI (gray), α4 and α5 are displayed in green and red, respectively. Immunostainings are shown in magenta (a, b, d, e–f, h) or in cyan (c, g). Arrows indicate examples of ectopic staining in the electroporated area. Arrowheads show examples of cells that performed apical extrusion. Scale bars in low magnification 80 µm, in zooms 50 µm. i, j Percentages of embryos with ectopic staining on the electroporated side per marker per experimental condition (i), across all markers per experimental condition (j). k Percentages of embryos with apical extrusion (cells located in the lumen). Embryos: α4 (n = 14), α5 (n = 9), α4 + α5 (n = 8). Representative images of embryos for fibronectin, pericentriolar material (PCM) 1, and N-cadherin are shown in Fig. S5. aPKC atypical protein kinase C. Source data are provided as a Source Data file.

Back to article page