Fig. 8: Condensins locally act to constrain nucleosomes at the mitotic chromosome axis. | Nature Communications

Fig. 8: Condensins locally act to constrain nucleosomes at the mitotic chromosome axis.

From: Single-nucleosome imaging unveils that condensins and nucleosome–nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes

Fig. 8

a Upper, cartoon depiction of nucleosomes inside or outside the chromosomal axis. Image 1, enhanced SMC2-mClover region, which corresponds to the axis; image 2, extracted axis region (yellow dashed line) and nucleosome dots. b MSD plot (±SD among cells) of TMR-labeled nucleosomes inside (axis, solid blue, n = 14) and outside (periphery, solid magenta, n = 14) the SMC2-mClover enriched axis regions of mitotic chromosomes in TSA-treated HCT116 cells (0.05 to 0.5 s). The MSD data without TSA treatment are shown as dashed lines [axis, blue (n = 18); periphery, magenta (n = 18)], which almost overlap the inside axis of TSA-treated chromosomes. ***, P ≤ 0.0005, Kolmogorov–Smirnov test (two-sided) for TSA peripheral versus TSA axis regions (P = 1.9 × 10−5). N.S. for TSA axis regions versus non-TSA treated axis (P = 0.33) or peripheral (P = 0.15) regions. MSD exponents from Supplementary Fig. 9a and AC values from Supplementary Fig. 9b are also shown in the brackets. c Nucleosomes around the periphery (those mostly free from condensins) in the TSA-treated chromosomes, whose nucleosome–nucleosomes interactions were weakened, were less constrained and had higher mobility than those around the axis.

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