Fig. 7: Effect of the rad50-C47 mutation on removal of hairpin structures from DNA ends.
From: Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms
A Hairpin formation from the IR-HO-17 construct after HO-induced DSB generation (B) Generation of a functional URA3 gene by homologous recombination between an IR cassette on chromosome VII and a donor on chromosome V after DSB induction. C URA3 recombination in cells containing the IR-17 cassette. Three independent experiments were performed and each value was plotted (asterisk). Numbers indicate the mean and standard deviation. D URA3 recombination in cells containing the IR-minus cassette. Three independent experiments were performed and each value was plotted (asterisk). Numbers indicate the mean and standard deviation. E URA3 recombination in cells containing the IR-4 cassette. Three independent experiments were performed and each value was plotted (asterisk). Numbers indicate the mean and standard deviation. F URA3 recombination in cells containing the IR-100 cassette. Three independent experiments were performed and each value was plotted (asterisk). Numbers indicate the mean and standard deviation.
