Fig. 3: PHF6 regulates histone acetyltransferase p300 and maintains chromatin accessibility.
From: PHF6 cooperates with SWI/SNF complexes to facilitate transcriptional progression
a Distribution of PHF6 occupancy across chromatin states in G401 RT cells (n = 2 independent biological replicates). b Co-immunoprecipitation of PHF6 with p300 from nuclear extracts of G401 RT cells (n = 3 independent biological replicates). c Western blot analysis p300 protein levels in nuclear extracts from G401 RT cells after knockdown of PHF6 (n = 3 independent biological replicates). d Density heatmap of chromatin occupancy of PHF6, p300, H3K14ac, and H3K27ac in shCTRL and shPHF6 conditions in G401 (ordered by PHF6 occupancy). e Density heatmap of significant losses (log2FC < 0, FDR < 0.05) and gains (log2FC > 0, FDR < 0.05) in accessibility and chromatin occupancy changes of H3K14ac and H3K27ac upon loss of PHF6 ranked based on PHF6 binding are shown in G401 cells (n = 3 independent biological replicates). Promoter regions are designated by the presence of H3K4me3. f Example loci reflecting loss of H3K14ac, H3K27ac, p300, and resultant accessibility at PHF6-bound promoters. Source data are provided as a Source Data file.
