Fig. 6: Laser emission for nucleolus in suspended cells.
From: Single-cell laser emitting cytometry for label-free nucleolus fingerprinting
a Single-cell microwell array on the mirror for co-localization analysis and the achieved nucleolus laser emission array. Scale bar: 40 μm. b Phase contrast image, fluorescence image and laser emission image of the fixed suspended A549 cells. Scale bar: 10 μm. c Corresponding spectra obtained from cells I, II, and III. d A live A549 cell in suspension displaying subtly visible nucleoli in the phase contrast image alongside the respective laser emission image. Scale bar: 10 μm. e Laser emission image observed across the entire cell under a pump energy ~ 143.5 μJ mm−2, with the nucleolar area exhibiting the highest laser intensity. Scale bar: 10 μm. f A typical Ince-Gaussian laser mode originating from an entire live suspended A549 cell. Scale bar: 10 μm. g Relationship between the laser spectral peak number and fluorescence-indicated nucleolus number when cells are in suspension (N = 64). h Correlation between lasing threshold and nucleolus diameter observed in both suspended and spreading cell states (mean ± SD, n = 13&13, 7&7, 7&7). i Lasing emission intensity of various-size nucleoli in live and fixed spreading cells (mean ± SD, n = 16&16, 12&12, 11&11). One-way analysis of variance (ANOVA) is used. Data are presented as box plots with median, minima, and maxima. Source data are provided as a Source Data file.
