Fig. 1: TADs containing developmental genes have distinctive features.

A TADs previously identified in mESC¹³ were classified based on their gene density in three different groups: High Density (HD), Medium Density (MD) and Low Density (LD). Then, the genes present within each TAD group were subject to GO enrichment analysis. For the MD (n = 17 enriched GO terms) and LD (n = 135 enriched GO terms) groups, only the top 10 most significantly enriched GO terms are highlighted (i.e. Q value ≤ 0.05), while for the HD group all the significantly enriched GO terms (n = 8) are presented. B The distribution of different groups of genes within TADs was investigated using previously generated TAD maps. TADs were divided in 10 bins of equal sizes, which were then grouped in five bin pairs based on their distance to the nearest boundary (e.g. Bin 1 is the closest to TAD boundaries; Bin 5 is the most distal from TAD boundaries) (see Methods). Bin 0 indicates genes located at inter-TAD regions (i.e. within TAD boundaries). C Distribution of different groups of genes (All (yellow; n = 21000 for mouse and 19288 for human; Housekeeping (HK; green; n = 3936 for mouse and 2779 for human); Developmental (Dev; purple; n = 962 for mouse and 1045 for human) within TADs according to Hi-C data previously generated in mESC^13,19 and hESC¹¹⁸. Heatmap plot (with scaling by rows) showing the distribution of developmental (D) and housekeeping (E) genes within TADs previously identified in several human (n = 37 TAD maps) and mouse (n = 11 TAD maps) cell types.