Fig. 7: Regulatory effects of Angiotensin II on aortic EndoMac progenitors.

a Surface expression of ACE, AGTR1 and AGTR2 on progenitors from aortic CFU-M of 12 w C57BL/6J mice (n = 6 mice). b ACE expression on progenitors in aortic digests from 12 w C57BL/6J mice (n = 4 mice). c ACE expression on progenitors from E9.5 YS CFU-M from C57BL/6J mice (n = 3 mice). d Normalized expression of Agtr1 and Agtr2 mRNA in progenitors from aortic CFU-M relative to donor-matched aortic digests from 12 w C57BL/6J mice (n = 6 mice; two-tailed Wilcoxon matched-pairs signed-rank tests). e Aortic CFU-M yield with different concentrations of AngII, normalized to no AngII control (n = 6, 12 w C57BL/6J mice; Friedman test with Dunn’s multiple comparisons test; *p = 0.04 and †p = 0.006 vs control). f Aortic CFU-M yield from 12 w C57BL/6J mice across serial passages in the presence or absence of 100 nM AngII (n = 4 mice). g Aortic CFU-M yield in the presence of inhibitors of ACE (Enalapril), AGTR1 (Losartan) and AGTR2 (PD123319) normalized to no inhibitor (control) (n = 7 mice; Friedman test with Dunn’s multiple comparisons test; *p = 0.02 for AGTR1 and AGTR2 inhibitors vs control). h Schematic of peritoneal transfer assay. GFP⁺ aortic progenitors were intraperitoneally injected into 12 w C57BL/6J mice with daily injections of PBS, AngII or M-CSF for 72 h. i Immunophenotype of GFP⁺ aortic progenitors before intraperitoneal injection. j Flow cytometry shows de novo formation of macrophages from GFP⁺ aortic progenitors under different conditions in peritoneal cavity after 72 h. No progenitor cells (Prog) negative control for GFP also shown. GFP green fluorescent protein. k Number of macrophages produced by progenitors under different conditions (n = 4 mice per condition; one-way ANOVA with Tukey’s multiple comparisons test; *p = 0.01 vs PBS). l–p mRNA expression of selected genes in aortic progenitors from 12 w C57BL/6J mice after treatment with AngII (AngII+). Expression normalized to β-actin and then to no AngII control (AngII-). Genes relate to (l) cell cycle, (m) progenitor/stem cell biology and self-renewal, (n) myelopoiesis, (o) macrophages, (p) endothelial biology and angiogenesis (n = 3 mice; two-tailed paired t-tests). Data summarized as mean ± SD. Source data are provided as a Source Data file. Figure 7 panel (h) created with BioRender.com released under a Creative Commons Attribution-Non-commercial-No Derivs 4.0 International license.