Fig. 1: The translational landscape of B. subtilis during sporulation reveals temporal clustering.
From: Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation
a Heatmap of the selected highly translated sporulation specific genes (mean values from two replicates of TPM ≥ 100, resulting in 633 genes). Highly translated genes from different sporulation regulons can be segregated into clusters of similar temporal activity and translation level based on k-means clustering. Data presented as log10(TPM + 1). b A summary heatmap of k-mean clusters constructed using Euclidean distance with complete linkage. c Scatter plots illustrating correlation between log2 transformed values of fold change between transcription and translation at the consecutive timepoints during sporulation. Mother cell specific regulons are σE in yellow and σK in red; forespore specific sigma regulons are σF in blue and σG in green. Best fit lines and Pearson R2 values describe the dynamics and correlation of fold change for transcriptome and translatome data. Source data are provided as a Source Data file.
