Fig. 1: Increased replication fork rate, not reduced origin activity, is the primary cause of PARPi on DNA replication. | Nature Communications

Fig. 1: Increased replication fork rate, not reduced origin activity, is the primary cause of PARPi on DNA replication.

From: DNA polymerase α-primase facilitates PARP inhibitor-induced fork acceleration and protects BRCA1-deficient cells against ssDNA gaps

Fig. 1

a Labeling scheme to evaluate the primary effect of PARPi in U2OS cells used in b and c. b DNA combing assay showing that APH reduces PARPi-induced fork acceleration. The scatter plot of fork rates based on IdU tract length is presented, with the mean values marked on the graph. Each dot represents one fiber; data were from three independent experiments (n = 3). Statistical analysis was conducted by Kruskal–Wallis test with Dunn’s multiple comparisons test. c Evaluation of origin activity by quantification of first-label origins showing that PARPi does not repress origin activity in the presence of APH. The mean values of three independent experiments (n = 3) with standard deviations indicated as error bars are shown. Statistical analysis was conducted by one-way ANOVA with Holm–Sidak’s multiple comparisons test. Source data are provided as a Source Data file.

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