Fig. 1: Generation and validation of Hnrnpr knockout mice.
From: hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis
a Survival analysis of Hnrnpr mutant mice. (Mean survival after 18 months: Hnrnpr+/+: 83.3% n = 22 mice; Hnrnpr−/−: 81.8%, n = 18 mice). Statistical analysis was performed using a Log-rank (Mantel-Cox) test. b Representative images of hnRNP R immunofluorescence in cultured motoneurons at DIV 6. Scale bar, 10 μm. The images are representative of three biological replicates. c Morphology of cultured DIV 6 motoneurons from Hnrnpr+/+ and Hnrnpr−/− mice immunostained with an antibody against tau. Scale bar, 50 µm. d SuperPlots of axon lengths of motoneurons. Statistical analysis was performed using an unpaired two-tailed Student’s t test. Data are mean ± s.d. of three biological replicates. Hnrnpr+/+, n = 629; Hnrnpr−/−, n = 477 motoneurons. e Quantification of motoneuron survival. Statistical analysis was performed using a two-way ANOVA with Tukey’s multiple comparisons test. ns, not significant. Data are mean ± s.d. of three biological replicates. Source data are provided as a Source Data file.
