Fig. 4: Axonal transcriptome alterations of hnRNP R knockout motoneurons are distinct from proteome changes.
From: hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis
a Scatter plot correlating logarithmized fold changes of transcripts differentially expressed (Padj < 0.05) in the axonal relative to the somatodendritic compartment of wildtype motoneurons from Briese et al.9 with this study. b, c Mean average (MA) plots showing somatodendritic and axonal transcriptome changes in Hnrnpr−/− relative to +/+ motoneurons cultured in microfluidic chambers from four biological replicates. Transcripts altered with Padj < 0.05 (b) or P < 0.05 (c) are highlighted. d Venn diagrams showing the overlap of deregulated (P < 0.05) transcripts in the somatodendritic and axonal compartments of Hnrnpr−/− motoneurons. e Box plots showing the number of iCLIP hits in transcripts differentially expressed in axons of Hnrnpr−/− relative to +/+ motoneurons cultured in microfluidic chambers from four biological replicates. Statistical analysis was performed using a Kruskal-Wallis test with Dunn’s multiple comparisons test. f Scatter plot correlating logarithmized fold changes of proteins and transcripts differentially abundant (P < 0.05) in axons of Hnrnpr−/− relative to +/+ motoneurons. g Venn diagrams showing the overlap of deregulated (P < 0.05) proteins with transcripts deregulated (P < 0.05) in the axonal compartment of Hnrnpr−/− relative to +/+ motoneurons.
